Electron microscopy of SARS-CoV-2 particles - Dataset 03

The dataset contains 122 transmission electron microscopy images of ultrathin (60-70 nm) plastic sections through extracellular SARS-CoV-2 particles in Vero cell cultures. Images were recorded with 1376 x 1032 pixel dimensions at 0.64 nm pixel size (12 bit) and stored in 16 bit TIF format. For visualization of the images, use an image viewer capable of reading 16 bit images (e.g. IrfanView). Image files are size calibrated and can be opened with the correct size calibration using ImageJ or Fiji using the Bioformats importer. The image files are accompanied by a PDF document which describes the methods which were used for generation of the images. The dataset was produced as dataset 03 for a comparative morphometric analysis of SARS-CoV and SARS-CoV-2. Further datasets which were used for the analysis are available in this repository (see dataset description document).

Related publication: Laue M, Kauter A, Hoffmann T, Möller L, Michel J, Nitsche A. Morphometry of SARS-CoV and SARS-CoV-2 particles in ultrathin plastic sections of infected Vero cell cultures. Sci Rep. 2021 Feb 10;11(1):3515. doi: 10.1038/s41598-021-82852-7. PMID: 33568700; PMCID: PMC7876034.

本数据集包含122张透射电子显微镜（Transmission Electron Microscopy）图像，成像对象为Vero细胞培养物中细胞外SARS-CoV-2病毒颗粒的超薄（60~70 nm）塑料切片。图像分辨率为1376×1032像素，像素尺寸0.64 nm，采用12位采集，以16位TIF格式存储。若需查看该系列图像，请使用支持读取16位图像的图像查看工具（例如IrfanView）。本数据集的图像文件已完成尺寸校准，可通过ImageJ或Fiji搭配Bioformats导入器，以正确的尺寸校准参数打开。本数据集附带一份PDF文档，详细说明用于生成该批图像的实验方法。本数据集作为数据集03，用于开展SARS-CoV与SARS-CoV-2的比较形态计量学分析。本分析所用的其他数据集可在本仓库中获取（详见数据集说明文档）。相关研究文献：Laue M, Kauter A, Hoffmann T, Möller L, Michel J, Nitsche A. 感染Vero细胞培养物超薄塑料切片中SARS-CoV与SARS-CoV-2病毒颗粒的形态计量学分析. Sci Rep. 2021年2月10日;11(1):3515. DOI: 10.1038/s41598-021-82852-7. PMID: 33568700; PMCID: PMC7876034.