Dataset for "Sex-dependent membranopathy in stored human red blood cells"

This Dataset contains the results of analysis of cold-stored, human packed Red Blood Cells (pRBCs). The blood was withdrawn from men N=12, aged <30 years (N=3), 30-39 years (N=4), >40 years (N=5) and women N=12, aged <30 years (N=4), 30-39 years (N=4), >40 years (N= 4). The measurements were conducted weekly for 8 weeks.
- 'Data for Figure 1' contains the results of biochemical analysis of (A) glucose, (B) lactate, (C) cholesterol, (D) triglycerides, (E) LDH, (F) free iron levels in pRBCs, performed with use of biochemical analyzer ABX Pentra 400 (Horiba Medical, Japan).
- 'Dataset for Figure 2' contains the results of flow cytometry analysis of surface protein expression in pRBCs, namely CD47 and Phosphatidylserine, performed with use of LSRII flow cytometer (BD Biosciences).
- 'Dataset for Figure 3' contains the results of flow cytometry analysis of Phosphatidylserine expression in pRBCs with regard to patients' age.
- 'Dataset for Figure 4A' contains the results of ektacytometry analysis of pRBC deformability, perforemd with use of RheoScanAnD 300 (RheoMeditech, Seoul, Korea) . Elongation Index was calculated on the basic of the geometry of the elliptical diffraction pattern: (A-B)/(A+B), where A is light, and W is the width of the deformed cell. El values were calculated for shear stress at 20 Pa.
- ' Dataset for Figure 4B' contains the results of complete blood count, comprising mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) mean corpuscular hemoglobin (MCH), red blood cell count (RBC), hemoglobin (HGB) and hematocrit (HCT) performed using a hematology analyzer Abc Vet (Horiba Medical, France).
- 'Dataset for Figure 5' contains the results of the atomic force microscopy (AFM) investigation of microparticle shedding from pRBCs, performed with use of WITec confocal CRM alpha 300 Raman microscope combined with an AFM (WITec, Ulm, Germany) . AFM images of 256 × 256 lines and 512 x 512 lines were collected in air conditions, from three different areas of 25 x 25 µm2, around ̴8 x 8 µm2 and 1.5 x 1.5 µm2. In order to determine the changes of RBCs surface and in situ formation of microparticles, cross sections of the AFM images were analyzed.

本数据集包含冷藏人类压积红细胞（packed Red Blood Cells，pRBCs）的分析结果。血液供者为12名男性与12名女性：男性群体中，年龄<30岁者3名、30~39岁者4名、>40岁者5名；女性群体中，年龄<30岁者4名、30~39岁者4名、>40岁者4名。所有检测每周开展1次，持续8周。
- ‘图1数据集’包含采用生化分析仪ABX Pentra 400（Horiba Medical，日本）完成的pRBC生化分析结果，检测指标包括(A)葡萄糖、(B)乳酸、(C)胆固醇、(D)甘油三酯、(E)乳酸脱氢酶（LDH）与(F)游离铁水平。
- ‘图2数据集’包含采用LSRII流式细胞仪（BD Biosciences）完成的pRBC表面蛋白表达流式细胞术分析，目标蛋白为CD47与磷脂酰丝氨酸（Phosphatidylserine）。
- ‘图3数据集’包含针对供者年龄分组的pRBC磷脂酰丝氨酸表达流式细胞术分析结果。
- ‘图4A数据集’包含采用RheoScanAnD 300（RheoMeditech，韩国首尔）完成的pRBC变形能力激光衍射分析结果。变形指数（Elongation Index）基于椭圆形衍射图样的几何参数计算，计算公式为(A-B)/(A+B)，其中A为变形细胞长轴，B为变形细胞短轴；变形指数值的计算条件为剪切应力20 Pa。
- ‘图4B数据集’包含采用血液分析仪Abc Vet（Horiba Medical，法国）完成的全血细胞计数结果，涵盖平均红细胞体积（mean corpuscular volume, MCV）、平均红细胞血红蛋白浓度（mean corpuscular hemoglobin concentration, MCHC）、平均红细胞血红蛋白量（mean corpuscular hemoglobin, MCH）、红细胞计数（red blood cell count, RBC）、血红蛋白（hemoglobin, HGB）与血细胞比容（hematocrit, HCT）。
- ‘图5数据集’包含采用WITec共聚焦CRM alpha 300拉曼显微镜联用原子力显微镜（atomic force microscopy，AFM）系统（WITec，德国乌尔姆）完成的pRBC微粒脱落原子力显微镜观测。该研究在空气环境中采集了256×256线与512×512线的AFM图像，采样区域涵盖3种不同尺寸：约25×25 µm²、~8×8 µm²与1.5×1.5 µm²。为分析红细胞表面变化与原位微粒形成情况，研究人员对AFM图像的横截面进行了分析。