The brain endothelial cell translatome analysis in the visual cortex at plastic and nonplastic ages. The brain endothelial cell translatome analysis in the visual cortex at plastic and nonplastic ages

In this study, we provide the translatome data in the brain endothelial cells (BECs) of the visual cortex at 3 different time points (pre-critical period (P10), critical period (P30), post-critical period (P100)) by using translating ribosome affinity purification (TRAP) combined with high-throughput RNA sequencing analysis in Tie2-Cre:Ribo-Tag mice, which are designed for expressing hemagglutinin A (HA)-tagged RPL22, a ribosomal protein in endothelial cells depending on CRE recombinase. To obtain a high-quality BEC pool, we modified the TRAP method by adding the capillary isolation process before the immunoprecipitation step and finally verified BEC-specific translating mRNA purification using RT-PCR. Overall design: The translatomic profiles of brain endothelial cells obtained from the visual cortex of mice at three different developmental stages (P10, P30, and P100).