Microarray profiling analysis of whole transcriptome expression in osteosarcoma

Osteosarcoma (OS)is a rare primary malignant bone tumor in adolescents and children with a poor prognosis. Identification of prognostic genes lags far behind the advance of the treatments. We identified differential genes by microarray analysis from paired OS tissues. Hub genes, gene set enrichment analysis, and pathway analysis were performed to gain an insight into the pathway alterations of OS. These results showed CPE could be served as a prognostic factor in osteoblastic OS and should be further investigated as potential therapeutic target. The present study evaluated the whole transcriptome expression of osteosarcoma progression and provided novel therapeutic targets for advanced osteosarcoma. This study was carried out on osteosarcoma tissues and adjacent normal tissue that were collected from the Depatment of Orthopaedic Oncology Surgery of the Beijing JiShuiTan Hospital. All of the patients received the four cycles of standard preoperative adjuvant chemotherapy (high dose methotrexate, ifosfamide, cisplatin and doxorubicin, MAP + IFO). The Agilent Human lncRNA Microarray 2019 (4*180k,Design ID:086188) was used in this experiment and data analysis of the 12 samples were conducted by OE Biotechnology Co., Ltd.