Replication Data for: Phaeoviruses Present in Cultured and Natural Kelp Species, Saccharina latissima and Laminaria hyperborea (Phaeophyceae, Laminariales), in Norway

This dataset contains data associated with the manuscript titled \"Phaeoviruses Present in Cultured and Natural Kelp Species, Saccharina latissima and Laminaria hyperborea (Phaeophyceae, Laminariales), in Norway.\", part of the ViraICE project (NFR-314108). The objective of the study was to determine the diversity, distribution, and prevalence of phaeoviruses in Norwegian kelps in the context of both coastal ecosystem management and food production in the aquaculture sector. To do so, we carried out a molecular analysis of cultured and wild samples of two economically important kelp species in Norway (Saccharina latissima and Laminaria hyperborea), along the Norwegian coast. The dataset named \"Kelp_sampling_data\" contains all sampling information (date, location, depth, seawater temperature, salinity, etc) related to the two kelp species harvested from 2016 to 2022; as well as the extracted DNA concentration (eluted in two different tubes, DNA_1 and DNA_2) and PCR results against the Phaeoviral MCP gene. The following parameters are described: - Sample - Species - Date - Location - Lat - Long - Sampling station - Depth (m) - Average temperature (Celsius) - Salinity - Dry weight (mg) - DNA 1 (ng/ul) - Volume (ul) - DNA 2 (ng/ul) - Volume (ul) - Virus positive? The total number of Phaoviral positive samples are as well described, per year and location/collector. The dataset named \"Kelp_sequencing_data\" contains all information obtained by sequencing some of the PCR positive samples (number of reads, number of matches against a reference sequence list, number of viral variants found...). The following parameters are described: - Sample ID - Species - Location - Date - ONT sequencing run ID - ONT sequencing barcode - # Sequences - # Virus variants refined - Virus variant (I, II, and/or III) - Read matches against reference sequences

本数据集关联一篇题为《挪威养殖与自然海带物种（Saccharina latissima及Laminaria hyperborea，褐藻纲、海带目）中的褐病毒（Phaeoviruses）》的手稿，隶属于ViraICE项目（项目编号NFR-314108）。本研究旨在结合沿海生态系统管理与水产养殖食品生产的背景，探明挪威海带中褐病毒的多样性、分布及流行率。为此，我们沿挪威海岸对当地两种经济价值重要的海带物种——长叶海带（Saccharina latissima）和掌状海带（Laminaria hyperborea）的养殖与野生样本开展了分子分析。
名为"Kelp_sampling_data"的数据集包含2016年至2022年采集的上述两种海带物种的全部采样信息（采样日期、采样地点、水深、海水温度、盐度等），以及提取的DNA浓度（分储于两个不同管中，即DNA_1与DNA_2）与针对褐病毒主要衣壳蛋白（MCP）基因的聚合酶链式反应（PCR）检测结果。该数据集涵盖以下参数：样本编号、物种、采样日期、采样地点、纬度（Lat）、经度（Long）、采样站位、水深（米）、平均温度（摄氏度）、盐度、干重（毫克）、DNA 1（纳克/微升）、体积（微升）、DNA 2（纳克/微升）、体积（微升）、病毒检测阳性与否。此外，本数据集还按年份、采样地点/采集者统计了褐病毒阳性样本的总数量。
名为"Kelp_sequencing_data"的数据集包含部分PCR阳性样本的测序相关全部信息（测序读段数、参考序列列表匹配数、检出的病毒变异株数量等）。该数据集涵盖以下参数：样本ID、物种、采样地点、采样日期、ONT测序运行编号、ONT测序条形码、序列总数、精修的病毒变异株数量、病毒变异株（I、II和/或III型）、与参考序列的读段匹配数。