Protective effect of vitreous against hemoglobin neurotoxicity

Hypothesis: Vitreous protects neurons from hemoglobin (Hb) toxicity. Model: Primary cortical cell cultures (murine); cultures treated with Hb 10 micromolar alone or with bovine vitreous or vitreous components. Outcome measures: Lactate dehydrogenase (LDH)release, propidium iodide fluorescence staining intensity, culture malondialdehyde (MDA), culture iron.研究假说：玻璃体（vitreous）可保护神经元免受血红蛋白（hemoglobin, Hb）毒性损伤。实验模型：小鼠源性原代皮层细胞培养物；将该细胞培养物分别以10微摩尔浓度的血红蛋白单独处理，或联合牛玻璃体或玻璃体组分进行处理。结局指标：乳酸脱氢酶（lactate dehydrogenase, LDH）释放量、碘化丙啶（propidium iodide）荧光染色强度、培养物丙二醛（malondialdehyde, MDA）含量、培养物中铁含量。