Development and Characterization of an Inducible Tensin1 Deficient Transgenic Murine Model

Tensin1 (TNS1) is a key component of focal and fibrillar adhesions, mediating fibrillogenesis, as well as the transduction of mechanical cues and adhesive signaling. To enable further TNS1 characterization, we have developed a novel transgenic mouse that allows for temporally controlled and lineage specific knockout of TNS1. We found no differentially observed effects of TNS1 knockout on mouse health, breeding capacity, or vital organ histopathology. In contrast, RNA sequencing analysis identified 171 differentially expressed genes with Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrating differential expression in osteoclast differentiation and a number of pathways involved in host immune response. Furthermore, STRING pathway analysis showed differential regulation of genes involved in B-cell and myeloid-related signaling pathways, a number of which were validated by reverse transcription-quantitative polymerase chain reaction (RT qPCR). These findings were corroborated by impaired function of TNS1 siRNA monocytes and increased detection of lymphocytes in the bronchoalveolar lavage within days following TNS1 knockout. This study provides a novel understanding of the phenotypic and genotypic changes that occur following knockout of TNS1 that may lead to subsequent understanding of its role in disease. Overall design: RNA sequencing profiling of TNS1 expressing (TNS1+/+) and inducible, conditional deletion TNS1 knockout mice (TNS1-/-) whole lung lysates. All animals were given tamoxifen for 7 days, and the left lung from each animal was flash frozen, lysated and processed for RNA sequencing 14 days after the completion of tamoxifen treatment.