Table 1 Microorganisms, identified with DOGE analysis. 16S rDNA sequences, obtained from sequencing ofDOGE bands, were assigned to the corresponding microorganisms@en

Microorganisms play an important role in the transformation of material within the earth's crust. The storage of CO2 could affect the composition of inorganic and organic components in the reservoir, consequently influencing microbial activities. To study the microbial induced processes together with geochemical, petrophysical and mineralogical changes, occurring during CO2 storage, long-term laboratory experiments under simulated reservoir P-T conditions were carried out. Clean inner core sections, obtained from the reservoir region at the CO2 storage site in Ketzin (Germany) from a depth of about 650 m, were incubated in high pressure vessels together with sterile synthetic formation brine under in situ P-T conditions of 5.5 MPa and 40°C. A 16S rDNA based fingerprinting method was used to identify the dominant species in DNA extracts of pristine sandstone samples. Members of the alpha- and beta-subdivisions of Proteobacteria and the Actinobacteria were identified. So far sequences belonging to facultative anaerobic, chemoheterotrophic bacteria (Burkholderia fungorum, Agrobacterium tumefaciens) gaining their energy from the oxidation of organic molecules and a genus also capable of chemolithoautotrophic growth (Hydrogenophaga) was identified. […]微生物在地壳内部的物质循环转化过程中发挥着关键作用。二氧化碳封存作业会改变储层内无机与有机组分的组成结构，进而对微生物活动产生影响。为研究二氧化碳封存过程中微生物介导的地球化学、岩石物理及矿物学变化与相关过程，研究团队开展了模拟储层压力-温度（Pressure-Temperature, P-T）条件的长期实验室实验。实验所用的洁净岩心内段采自德国凯津（Ketzin）二氧化碳封存场址储层区域约650米深度处，将其与无菌合成地层水一同置于高压容器中，在5.5 MPa、40℃的原位温压条件下进行培养。研究采用基于16S rDNA的指纹图谱法，对原始砂岩样品的DNA提取物中的优势菌种进行鉴定。已鉴定出变形菌门（Proteobacteria）α-、β-亚群以及放线菌门（Actinobacteria）的相关菌株。截至目前，已获取两类细菌的序列：一类是通过氧化有机分子获取能量的兼性厌氧化能异养细菌——真菌伯克霍尔德菌（Burkholderia fungorum）与根癌农杆菌（Agrobacterium tumefaciens），另一类是具备化能自养生长能力的食氢菌属（Hydrogenophaga）。[…]