Hedgehog-regulated genes in the chick coelomic epithelium

In the experimental group, the coelomic cavity of HHStage 12-13 chick embryos was electroporated with a plasmid expressing PatchedDLoop2 (a dominant negative hedgehog pathway inhibitor) and GFP. In the control group, a plasmid expressing GFP alone was electroporated. After 14 hours, embryos were dissociated into single cells, FACS sorted, and processed for RNAseq. 5 experimental and 4 controls were analyzed. These were all separate biological replicates.