CIL:41364, Mus musculus, adenocarcinoma. In Cell Image Library

The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 15.6 nm thick slice of the computed tomogram in the x-y plane, and shows a region of the nuclear envelope showing a 'bleb'. The nucleus interior is an lower right. The images have a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation),he latter corresponding to~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group. See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.

本图像展示了基于X射线显微镜（x-ray microscopy）获取的小鼠腺癌细胞三维断层重建切片。活细胞通过浸入液态乙烷的快速冷冻（plunge freezing）实现冷冻固定，随后对该玻璃化样品采用无染色方式进行X射线显微镜观测，所用X射线波长可在水与有机物质之间形成高对比度。图像采集时，样品倾斜角度覆盖-60°至+60°区间，步长为1°。本图像为计算机断层扫描结果在x-y平面上的15.6 nm厚度切片，可见核被膜区域存在一处“核膜凸起（bleb）”。细胞核内部位于图像右下角。该图像的分辨率约为36 nm（瑞利判据（Rayleigh Criterion））与70 nm（傅里叶环相关法（Fourier Ring Correlation）），后者对应约35 nm的半间距分辨率。本CIL数据集组还收录了该研究的其他X射线图像。另可参阅：W G Muller等学者2012年发表于J Struct Biol 177:179-192的《基于软X射线断层扫描构建哺乳动物细胞超微结构图谱》一文。