Accuracy and precision of 10H10C/6G6B and 10H10C/2C4B AlphaLISAs on HtrA3 standard.

Concentrations of HtrA3 (HtrA3-L-S305A) were back calculated from the HtrA3 calibration curve and the inter- and intra-assay variations determined using AlphaLISAs in IB and analyte-depleted human serum matrix respectively. Results are from five independent experiments; three assays in triplicate and two assays in ten replicate wells. The intra-assay variation was calculated from one assay of ten replicate wells.