Combinatorial effect of ARTP mutagenesis and ribosome engineering on an industrial Streptomyces albus strain S12 for enhanced biosynthesis of salinomycin

Streptomyces albus S12, TK and Tet30Chl25 are the parental strain , low-yield and high-yield of salinomycin mutant obtained by ARTP and ribosome engineering ,respectively. There are total 1602 differentially expressed genes (DEGs) show differences in expression between the mutant strain TK, Tet30Chl25 and the initial strain S12. KEGG pathway analysis of differentially expressed genes (DEGs) between the mutant strain TK, Tet30Chl25 and the initial strain S12 show that the relevant differential pathways affecting salinomycin production were mainly related to butanoate metabolism, starch and sucrose metabolism, glyoxylate metabolism. Besides , the transcription of genes in the salinomycin biosynthesis gene cluster and the transcription level of related genes in the precursors biosynthesis pathway were more active in the high-yield salinomycin production strain Tet30Chl25. Furthermore, the transcription level ribosomal protein, string response, two component system and sigma factors are more active in high-yield of salinomycin mutants and that may involve in regulation of salinomycin biosynthesis and may account for the high-yield of salinomycin. Overall design: Expression profiles from Smutant TK, Tet30Chl25 and the parental strain strain were obtained by an Illumina HiSeq™2000 sequencing machine from samples taken at five day points in fermentation medium (0.8% germ powder, 0.5% soybean powder, 0.2% potassium chloride, 0.10% sodium chloride, 0.16% urea, 0.2% tartaric acid, 0.01% magnesium sulfate, 0.01% dibasic potassium phosphate, 0.5% calcium carbonate, 15% soybean oil, pH 6.6–6.9).