A list of putative TCS components found in the transcriptome of differentiating barley endosperm transfer cells (ETCs) by 454 sequencing.

Sequence information was complemented using public available EST databases and genomic sequences generated at the IPK Gatersleben. Prediction of coding sequences and protein sequences was done using FGENESH (http://linux1.softberry.com) and Augustus gene prediction (http://augustus.gobics.de) web-based interfaces. $noted domains are histidine kinase domain (HK), receiver domain (REC), GAF domain which is implicated in ethylene binding, CHASE domain for cytokinin binding, conserved histidine-containing phosphotransfer domain (HPt), Myb-like DNA binding domain (MYB), CCT motif found in clock proteins (CCT); brackets mark diverged domains and indicate changed amino acids, *not present in HarvEST 35, **not present in full-length cDNA collection of spring barley, #inferred amino acid sequences were compared with protein sequences computed in the Rice Genome Annotation project using the BlastP tool with the default settings, §full-length protein sequence.