CHLOROPHYLL A CONCENTRATION collected from NOAA Ship OSCAR ELTON SETTE in Hawaii EEZ and Papahanaumokuakea Marine National Monument from 2013-05-08 to 2013-06-03 (NCEI Accession 0155964)

Water samples were collected from the ocean surface using a bucket and from below the surface using bottles attached to the CTD during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID SE 13-03). A minimum of three surface water samples were taken each day, primarily at 0900, 1200, and 1500 hours local ship time. Surface water samples were also collected opportunistically during some cetacean sightings. CTD samples were collected once each morning.

The 250ml water samples were filtered onto GF/F filters, placed in 10ml of 90% acetone, refrigerated or frozen for 24 hours, and then analyzed for chlorophyll a concentration using the Turner Designs model 10AU field flourometer. Measurements were recorded in an Excel spreadsheet.

本数据集的水样采集自美国太平洋岛屿渔业科学中心（Pacific Islands Fisheries Science Center）鲸类研究项目的船舶鲸类调查航次（航次编号SE 13-03）：表层海水样本通过水桶采集，次表层海水样本则通过搭载于CTD（Conductivity-Temperature-Depth，温盐深仪）的采水器采集。每日至少采集3份表层水样，采样时间主要为当地船时的09:00、12:00与15:00；部分鲸类目击事件期间，也会机会性采集表层水样。CTD采水样每日清晨固定采集一次。

每份250ml的水样经GF/F滤膜过滤后，转移至10ml体积分数为90%的丙酮溶液中，经冷藏或冷冻保存24小时后，使用Turner Designs 10AU型野外荧光光度计（Turner Designs model 10AU field fluorometer）完成叶绿素a浓度的测定分析。所有测定数据均记录于Excel电子表格中。