Gene expression profile at single cell level of Basal Cells (BCs) from nasal scrapings of patients with and without cystic fibrosis

Respiratory epithelium interact with our microbiome as well as environmental bacteria, and are critical in maintaining homeostasis in face of disruption such as injury or infection. Here we investigate the impact of a filamentous bacteriophage on responses of these cells to bacterial stimulus. Overall design: Single-cell suspensions of basal cell culture were prepared by lifting adherent cells using trypsin digestion. Dead cells were removed using the MACS Dead Cell Removal kit (Miltenyi Biotech). Cells were then washed and counted as per manufacturer's instructions and libraries were prepared using the 10X NEXT GEM 3' Gene Expression Library scRNA Seq v3.1 reagents and workflow (10x Genomics). Library concentration and quality was confirmed by BioAnalyzer. Libraries were sequenced on the HiSeq PE150 platform.