Raw Data for QRT-PCR Screen

Data are shown as Ct, the number of cycles required for amplification of a particular target gene from a cDNA preparation. Changes in gene expression in the nhr-49(nr2041) mutant were measured as ΔCt. ΔCt are obtained by subtracting nhr-49(Ct) from N2(Ct) and adjusting for RNA concentration differences with a correction factor (CF). The CF was determined by aligning the data to a set of standard genes. Thus the equation for ΔCt is as follows: Genes were designated as nhr-49 dependent if the Ct was >1.0 in multiple developmental stages (and was greater than the standard error). nhr-49 targets are marked with an X. The QRT-PCR results of the major nhr-49 targets, acs-2, ech-1, fat-5, and fat-7, were confirmed with a second primer pair. (100 KB XLS).