Changes in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitro. Changes in the biologica function of necroptotic, peripheral blood mononuclear cells under irradiation in vitro

The secretome of y-irradiated peripheral blood mononuclear cells (PBMC) is well known for its pleiotropic effects on tissue protection and regeneration. Irradiation of PBMC lead to necroptosis of a certain amount of PBMC in a cell-sorter analysis. The aim of this study was to investiage the trancriptomic changes of PBMC and PBMC subsets (CD4, CD8, CD19, CD56, CD14) in response to y-irradiation Overall design: In total 72 samples obtained from four healthy donors were analysed (0h= cells immediately after purification, 24h_0Gy = cells cultivated for 24 hours without prior radiation; 24h_60Gy = cell cultivated for 24 hours after irradiation with 60Gy). At each time point six different cell populations were analysed (unseparated PBMC, and separated PBMC subsets: CD4, CD8, CD14; CD19; CD56).