RNA m6A methylation in unilateral ureteral ligations model

Chronic kidney disease (CKD) is a high incidence disease. Renal fibrosis
is the ultimate pathological pathway for chronic kidney diseases leading
to end-stage renal failure. There is currently no effective treatment to
prevent the renal fibrosis. Renal EMT is a key mechanism of renal
fibrosis. Recent evidence suggests m6A modification in RNA can regulate
EMT in tumor cells. However, a role of m6A RNA modification in renal
fibrosis has not been reported. Therefore, we investigate whether m6A RNA
methylation plays an important role in the epithelial-to-mesenchymal
transition (EMT) process of renal fibrosis. Unilateral ureteral
ligations were performed in C57BL/6 mice to establish a mouse model of
renal fibrosis and TGF-b intervention was used in HK2 cells to
establish a cellular model of renal fibrosis. The effects of m6A and the
role of Mettl14 on kidney disease and HK2 cells were
studied.