Identity of differentially expressed proteins from pooled <i>P. xylostella</i>-resistant or –susceptible <i>A. thaliana</i> Rils.

2D-PAGE protein profiles from pooled P. xylostella-resistant or –susceptible A. thaliana Rils were compared and differentially expressed proteins identified. Spot numbers refer to Fig. 2. ‘Gene’ refers to the unique identifier in the A. thaliana database (www.arabidopsis.org). Identified proteins that were differentially expressed by 2-fold or greater are listed here. The fold difference represents the mean on the three gels using different biological samples (n = 3). P-value refers to the significance of the difference in intensity for each spot between R and S Rils following the application of a t-test. a) For ‘R’ spots, protein was more abundant in the ‘Resistant’ sample compared to the ‘Susceptible’ one. For the ‘S’ spots, the converse applied. b) Mass/pI gained from NCBI BLAST. All other mass/pI values came from TAIR or Mascot. c) Protein spot absent from ‘Susceptible’ sample, thus fold difference cannot be given. d) It was not possible to differentiate between these possible proteins by MS/MS, since only one peptide was detected in spots and this corresponds to a common region in the protein sequence. However, this is the likely correct identification – see ‘Results and Discussion’. e) Likely correct identification – see ‘Results and Discussion’. f) One peptide identified through manually sequencing the mass spectra. g) Protein spot absent from ‘Resistant’ sample, thus fold difference cannot be given.