OME-Zarr Fractal multiwell plate with segmentation & measurements

Example dataset of human induced pluripotent stem cells, imaged at 40x magnification with a Yokogawa CV7000. This dataset has been converted to OME-Zarr & processed using the included workflow for the Fractal platform: https://fractal-analytics-platform.github.io/ It contains 23 wells of a cardiac differentiation time course. The segmentation algorithm has been optimised for the undifferentiated cells and segmentation quality is known to be worse on denser wells. 3 Channels were imaged: - C01: DAPI, nuclear stain - C02: nanog, antibody staining with Bio-Techne AG, AF1997-SP, Lot KKJ0617121 for the stemness marker nanog - C03: Lamin B1, antibody staining with Abcam, ab16048, Lot GR3244890-2 for the nuclear envelope marker Lamin B1 This dataset is a maximum intensity projection of 3D data that was acquired in the Pelkmans lab in August 2020.本数据集为人类诱导多能干细胞（human induced pluripotent stem cells）示例数据集，采用横河（Yokogawa）CV7000成像系统以40倍放大倍率拍摄。该数据集已转换为OME-Zarr格式，并通过配套工作流针对Fractal平台进行了处理，相关平台地址为：https://fractal-analytics-platform.github.io/ 数据集包含23个孔板的心脏分化时间序列样本。本分割算法已针对未分化细胞优化，已知在细胞密度较高的孔板中，分割质量有所下降。 本次成像共包含3个通道： - C01：DAPI，细胞核染色剂 - C02：Nanog（nanog，干性标志物），采用Bio-Techne AG公司的AF1997-SP抗体进行免疫染色，批次号KKJ0617121 - C03：核纤层蛋白B1（Lamin B1，核被膜标志物），采用Abcam公司的ab16048抗体进行免疫染色，批次号GR3244890-2 本数据集为2020年8月于Pelkmans实验室采集的三维数据的最大强度投影结果。