Transcriptome analysis of cells carrying Type II Csp231I restriction-modification (RM) system reveal the crosstalk between two unrelated transcription factors - C protein and Rac prophage repressor

Our transcriptome analysis supported by other in vivo and in vitro assays showed that C protein can directly silence the RacR repressor and thus affect the Rac prophage-related genes. Overall design: Cultures of E. coli MG1655 carrying following plasmids: pMCsp231 (additional protective MTase gene on ACYC177 derivative), and plasmid with RMs of interest: WT (p18) and two variants: C-R+ (p30) and C+R- (p24), were grown in LB medium.