Phages enhance both phytopathogen density control and rhizosphere microbiome suppressiveness

Bacteriophages, viruses that specifically target plant pathogenic bacteria, have emerged as a promising alternative to traditional agrochemicals. However, it remains unclear how phages should be applied to achieve efficient pathogen biocontrol, and to what extent their efficacy is shaped by indirect interactions with the resident microbiota. Here we tested if the phage biocontrol efficacy of Ralstonia solanacearum phytopathogenic bacterium can be improved by increasing the phage cocktail application frequency, and if the phage efficacy is affected by pathogen-suppressing bacteria already present in the rhizosphere. We find that increasing phage application frequency improves R. solanacearum density control, leading to a clear reduction in bacterial wilt disease in both greenhouse and field experiments with tomatoes. The high phage application frequency also increased the diversity of resident rhizosphere microbiota and enriched several bacterial taxa that were associated with the reduct..., , , # Phages enhance both phytopathogen density control and rhizosphere microbiome suppressiveness

[https://doi.org/10.5061/dryad.dz08kps40](https://doi.org/10.5061/dryad.dz08kps40)

## Description of the data and file structure

Datasets included:

### Figure 1

* In the row of “trt”, “0” represents no phage cocktail application, “1” represents applied once (day 2), “2” represents applied two times (days 2 and 9), “3” represents applied three times (days 2, 9 and 16) after pathogen inoculation. \"trt\" means treatment.
* In the row of “rep”, “1” represents the first replicate, “2” represents the second replicate, “3” represents the third replicate, “4” represents the fourth replicate.
* “index” is the mean disease incidence of 6 plants within each replicate.
* “audpc” is the area under the disease index curve, calculated as the integral.

### Figure 2

* The alpha diversity metrics, Species Richness, and Simpson Index were calculated using the 'diversity' function within the R pac...

噬菌体（Bacteriophages）是一类特异性靶向植物病原细菌的病毒，现已成为传统农用化学品（agrochemicals）极具潜力的替代方案。然而，目前仍未明确噬菌体的最优施用方式以实现高效的病原物生物防控，且其防控效果受与土著微生物群间接互作的调控程度尚不清晰。本研究旨在验证两个科学问题：一是通过提升噬菌体鸡尾酒（phage cocktail）的施用频率，是否可增强青枯雷尔氏菌（Ralstonia solanacearum，植物病原细菌）的噬菌体生物防控效果；二是噬菌体的防控效果是否会受到根际（rhizosphere）中已存在的病原抑制细菌的影响。

研究结果表明，提升噬菌体施用频率可强化对R. solanacearum的密度调控，在番茄的温室与田间试验中均显著减轻了青枯病的发生程度。高频率施用噬菌体同时提升了土著根际微生物群的多样性，并富集了多种与……相关的细菌类群。
# 噬菌体可同时增强病原细菌密度调控与根际微生物群抑病能力
https://doi.org/10.5061/dryad.dz08kps40

## 数据集与文件结构说明
包含以下数据集：

### 图1
* 在"trt"（处理组，treatment）列中，"0"代表未施用噬菌体鸡尾酒，"1"代表病原菌接种后施用1次（第2天），"2"代表施用2次（第2天与第9天），"3"代表施用3次（第2天、第9天与第16天）。
* 在"rep"列中，"1"代表第1次重复，"2"代表第2次重复，"3"代表第3次重复，"4"代表第4次重复。
* "index"代表每个重复中6株植株的平均病害发病率。
* "audpc"为病害指数曲线下面积，通过积分法计算得到。

### 图2
* 物种丰富度（Species Richness）与辛普森指数（Simpson Index）等α多样性（alpha diversity）指标，通过R语言包中的"diversity"函数计算得到。