Characterization of RNAs in soybean (Glycine max)

RNA-seq was used to characterize gene expression in soybean from a wide range of tissues. The primary focus of the project was small RNAs, and the identification of microRNAs and phased siRNA-generating loci, but RNA-seq data were generated from the same samples. This project was supported by the United Soybean Board. mRNA profiling was done by Illumina TruSeq sample preparation (not stranded) followed by high-throughput sequencing using an Illumina HiSeq 2500 at the Delaware Biotechnology Institute. The study focused on small RNA, with these RNA-seq libraries made from the same samples (see PMID: 25465409).