Rabbit vaccination groups.

Male and female NZW rabbits received immunizations at three week intervals either sequentially or in combination into different quadricep muscles. The reduced quantity of GLA-AF when compared with the murine experiments more closely models the amount that will be administered in humans, a refinement based on our repeat experiments where we found that immune stimulation elicited by 5 ug GLA-AF was equivalent to 20 ug GLA-AF. The regime-shortened group DNA-MVA/gp140+GLA animals were euthanized at week 21, while the sequential immunisation and control animals were euthanized at week 29, to fulfil the requirement for toxological analysis.