Collection of rice-root associated bacteria sampled in Burkina Faso, 2017-2019

In Burkina Faso, studies have been initiated on the diversity of rice rhizosphere and root microbiomes using metabarcoding (Barro et al., 2022) and culturable bacteria (Sondo et al., 2023) approaches to analyse the effects of rice production systems (irrigated cropping areas vs rainfed lowland conditions) on microbiome diversity. As a continuity of these studies we aimed to develop a large collection of culturable rice-associated bacteria from different rice fields in Burkina Faso to serve as a valuable genetic resource for screening biostimulant and biocontrol strains. In this regard, root samples were collected in rice fields over three consecutive years (2017 to 2019) at 22 sites in two studied rice growing regions in western Burkina Faso (Barro et al., 2021; Barro et al., 2022), and at different rice plant development stages. Phenotypically distinct bacterial colonies obtained from whole roots (total microflora) or from surface sterilised roots (putative endophytes) and subsequently plated on five different culture media (TSA of 10 and 50%, NGN, FNb, Baz, PCAT) were reisolated for further taxonomic and phenotypic characterization. Over 3,800 rice root-associated bacteria were isolated from five bacterial culture media. A working collection of 1,013 isolates was characterized at the taxonomic (by 16S fragment sequencing) and phenotypic (in vitro PGP capacity) levels. The results obtained for 1013 isolates are presented in the table as metadata concerning isolate identity, sampling location, sampling date, 16S rDNA accession numbers and sequences, in vitro PGP capacities.