Additional file 1 of Effect of the dietary supplementation with extracts of chestnut wood and grape pomace on performance and jejunum response in female and male broiler chickens at different ages

Additional file 1. RNA-seq libraries sequenced and transcriptional changes. Averages of traits for jejunum morphology and immuno-histochemical analyses according to interactions between sex and age and between sex and diet are provided in Table S1 and Table S2, respectively. Details on sequenced RNA-seq libraries are provided in Table S3. Comparisons among transcriptional profiles of same-sex and same-age chickens fed CN and GP diets compared to those of chickens fed the C diet, thus identifying significant DEGs, are shown in supplementary Tables S4, S5 and S6. Annotated genes significantly regulated in more than one comparison are listed in Tables S5. Gender-related and sex-related transcriptional changes are reported in Tables S5 and S6. Functional enrichment analysis is shown in Tables S7.

附加文件1：已测序RNA-seq文库与转录组变化。按性别与年龄、性别与日粮的交互效应分组的空肠形态学与免疫组织化学分析（immuno-histochemical analyses）的性状平均值，分别见于附表S1和附表S2。已测序RNA-seq文库的详细信息见于附表S3。将饲喂CN日粮与GP日粮的同性同龄鸡的转录组谱，与饲喂C日粮的鸡的转录组谱进行对比以筛选显著差异表达基因（Differentially Expressed Genes，DEGs），相关对比结果见于补充附表S4、S5和S6。经多次对比分析得到显著调控的注释基因见于附表S5。与性别相关的转录组变化情况见于附表S5和S6。功能富集分析（Functional enrichment analysis）结果见于附表S7。