Fluorescence emission spectra of Laurdan in phase-separated solid-supported lipid bilayer excited with azimuthally, radially and linearly polarized beams

Fluorescence emission spectra of Laurdan in liquid-ordered and liquid-disordered phases in solid-supported lipid bilayer exhibiting coexisting phases, excited with azimuthally, radially and linearly polarized laser beams. Lipid bilayer was composed of an equimolar mixture of 1,2-dimyristoleoyl-sn-glycero-3-phosphocholine, cholesterol, and egg yolk sphingomyelin. Mica was used as a solid support. Laurdan was excited with a picosecond laser diode with λ = 375 nm (PicoQuant). Spectra were collected with a spectrograph Kymera 328I-C (Andor) equipped with an EMCCD camera iXon Ultra 888 (Andor).本数据集涵盖了具有共存相的固相支撑脂质双层（solid-supported lipid bilayer）中，劳丹素（Laurdan）分别处于有序液相（liquid-ordered phase）与无序液相（liquid-disordered phase）时的荧光发射光谱，实验采用方位偏振、径向偏振与线偏振激光束对样品实施激发。该脂质双层由等摩尔比混合的1,2-二肉豆蔻油酰-sn-甘油-3-磷酸胆碱、胆固醇与蛋黄鞘磷脂配制而成，以云母作为固相支撑基底。劳丹素的激发光源为波长λ=375 nm的皮秒激光二极管（PicoQuant）。光谱数据通过安道尔（Andor）公司的Kymera 328I-C光谱仪采集，该系统搭载有安道尔公司的iXon Ultra 888电子倍增电荷耦合器件（EMCCD）相机。