Raw data for Figure 4B: Pre-stimulation with IFN-γ, TNF-α and IL-1β does not enhance the immunosuppressive effect of Mesoangioblasts/HIDEMs
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https://f1000.figshare.com/articles/dataset/Raw_data_for_Figure_4B_Pre_stimulation_with_IFN_TNF_and_IL_1_does_not_enhance_the_immunosuppressive_effect_of_Mesoangioblasts_HIDEMs/106728/1
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HIDEMs/mesoangioblasts were left untreated or were stimulated with IFN-γ, TNF-α or IL-1β (20ng/ml) for 24h before setting up co-cultures with CFSE labelled PBMC and anti CD3/CD28 beads. After 6 days cells were harvested and surface stained for CD3 and 7AAD before analysis of CFSE dilution. CD3+CFSE diluted cell numbers were calculated using counting beads as before. Experiments were carried out in duplicates. n=4.
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2016-01-12
