Gene expression profiles of stromal cells in ovarian cancer co-culture models. Homo sapiens

Goal: to profile gene expression change that occur when cancer-associated firboblast precursor cells are co-cultured with normal epithelial cells (IOSE4) or epithelial ovarian cancer cells (HeyA8) Overall design: MSCs and INOF cells were co-cultured with IOSE4 cells or Hey.A8 EOC cells. 1000 stromal cells were plated into 6 well plates and 10,000 epithelial cells plated into porous inserts (pore size 1μm, Greiner BioOne). After 24hrs, cells were refed and epithelial cell inserts added to the stromal cell cultures. Cells were grown in 50% INOF medium and 50% MSC medium for 7 days before harvesting RNA