Editing profile of OPHN1 in astrocytoma cell lines overexpressing ADAR2 or silenced for ADAR1.

Editing analysis of OPHN1 pre-mRNA (AluJo, sites 1–14) in U118 and U87 astrocytoma cell lines overexpressing ADAR2 or silenced for ADAR1. The sites preferentially edited by a specific enzyme are indicated in bold and the sites edited by both ADARs are in bold and underlined. The sites 7-8-9 are mainly edited by ADAR1 (as a strong decrease of editing values at these sites is visible after ADAR1 silencing, whilst ADAR2 overexpression does not cause modifications). The sites 3-4-10 are specifically edited by ADAR2 (as a significant increase of editing percentages at these sites is visible in ADAR2 overexpressing cells, whilst no change appears after ADAR1 silencing). Sites 1 and 11 are significantly modulated by both the ADAR enzymes. The RNA editing levels are expressed as percentages (mean ± s.e.m, n = 3). nd = not determined.