Microbial Community Diversity of Jinghong Laying Hens at Peak Production Based by 16S rRNA Sequencing

In this study, we evaluated the phylogenetic diversity within the duodenum (S), jejunum (K) and cecum (M) of the three different intestinal gut sections microbiota of 30-week-old Jinghong laying hens. The bacterial DNA was sequentially isolated and the V3 to V4 regions of 16S rRNA genes were amplified. The amplicons were then subjected to high-throughput sequencing to analyze the bacterial diversity of different groups.