Cardiac FGF21/FGFR1 expression levels in secretion of FGF21 in response to ischemia and obesity: reduction in βKlotho and MAPK/PI3k-Akt signalling in obese hearts.

Using rat heart Langendorff model and inducing global ischemia for 30[Fig. 5A1], protein [Fig. 5A2], and secretion of FGF21 in Langendorff coronary exudates [Fig. 5A3] were measured. Similarly, changes in FGFR1 mRNA expressions [Fig. 5A4] were measured. Data shown are means ± SEM of triplicates. The values represented are relative to basal. ***PPFig. 5B1], protein [Fig. 5B2], FGF21 secretion (in Langendorff coronary exudates) [Fig. 5B3] and FGFR1 mRNA [Fig. 5B4] expressions were measured in obese and lean rat hearts. Graphical representation of a key signalling component of FGF21/FGFR1; βKlotho protein level in obese and lean hearts [PFigure 5C1]. Graphical representation of ERK1/2 [Fig. 5C2], Akt [Fig. 5C3] and AMPK [Fig. 5C4] phosphorylation levels in lean and obese hearts with FGF21 (100 nM) pre-infusion. Data shown are means ± SEM of triplicates. The values represented are relative to basal. *P**PNS-non-significant; n = 6 per group.