The Golden Gate strategy entails the use of type IIS restriction enzymes, which cut outside of their recognition sequence. It enables unrestricted design of unique DNA fragments that can be readily an
We developed a methodology for generating reusable and modular integration-based combinatorial libraries and demonstrate their usefulness by engineering red beet pigment production in the yeast Saccha
A precisely designed synthetic library comprising 244,000 variants of a 96 nucleotide sequence fused to a fluorescent reporter was build to unravel the determinant of translation efficiency in E. coli
We describe a novel method for constructing pools of DNA sequences that encode large proteins with molecular diversity. Sets of primer pairs that form 8 to 10 complementary base pairs in the 3′ region
