Streptomyces from rhizospheres and compost

This information was obtained in order to demonstrate the presence of actinobacteria in different rhizospheres and in a compost system, which would inhibit the growth of bacteria resistant to some antibiotic. In addition, the aim of this study was to identify by molecular methods and phylogenetic analysis the species of native bacteria with antimicrobial activity, mainly of the Streptomyces genus, recovered from these environments.

In these data it is possible to see the number of isolates obtained from different rhizospheres and from a composting system, the culture media used for their isolation and the antimicrobial activity against antibiotic resistant and non-resistant bacteria; this activity was initially measured by direct antagonism by perpendicular streak technique. In the photo of the preliminary test, some isolates with inhibitory potential can be identified according to the space without growth between the streak of the native organism and the streak of the control bacteria.

An initial enzyme profile of all the isolates obtained is also presented, which allowed the physiological characterization of native Streptomyces with antimicrobial activity. A biochemical analysis was carried out through conventional identification tests and the microscopic and macroscopic morphological characteristics are reported, the latter analyzed through a comparison of the color of the colonies obtained in different culture media.

The data also shows the percentages of inhibition obtained with the active isolates against bacteria resistant to antibiotics. These calculations were made through the perpendicular streak method comparing the area that presented growth of the control microorganism and the areas where it was inhibited.

The native isolates that showed activity were identified through molecular tests and phylogenetic analysis, for which sequences reported in GenBank for the 16S ribosomal gene were organized and shown in the respective sequence bank file.

本研究信息旨在证明不同根际环境及堆肥系统中存在放线菌，这类放线菌可抑制部分抗生素耐药细菌的生长。此外，本研究的目标为通过分子生物学方法与系统发育分析，鉴定从上述环境中分离得到的、具有抗菌活性的本土细菌物种，其中主要涵盖链霉菌属（Streptomyces）类群。

本数据集包含从不同根际环境与堆肥系统中分离得到的菌株数量、分离所用培养基、各菌株对抗生素耐药与非耐药细菌的抗菌活性数据；该抗菌活性最初通过垂直划线直接拮抗法进行测定。在预实验图像中，可通过对比本土菌株划线区与对照细菌划线区间的无生长空白区域，识别具备抑菌潜力的分离菌株。

本数据集同时收录了所有分离菌株的初始酶谱特征，以此完成对具有抗菌活性的本土链霉菌（Streptomyces）的生理特性表征。研究通过常规鉴定试验完成生化分析，并记录了菌株的显微与宏观形态特征；其中宏观形态特征通过比对不同培养基上菌落的颜色进行分析。

数据集还展示了活性菌株对抗生素耐药细菌的抑菌百分比数据；该抑菌百分比通过垂直划线法计算得出：对比对照微生物的生长区域与被抑制区域的面积，即可得到对应抑菌百分比。

通过分子试验与系统发育分析，本研究对具备抗菌活性的本土分离菌株进行了物种鉴定，本次鉴定所用的16S核糖体基因(16S ribosomal gene)序列取自GenBank(GenBank)数据库，并已整理至对应序列库文件中。