Polysome profiling RNAseq of cells transfected with an oligonucleotide targeting the ES6S region of the 40S subunit

Polysome profiling experiments were carried out to identify the mRNAs that have altered the translation efficiency (counts in polysomal fraction/counts in monosomal fractions) in the presence of an oligonucleotide targeting a specific region of the ribosomal RNA, to study the implication of this region in the translation of specific mRNAs Polysome profiling experiments of cuture cells transfected with the oligonucleotide of study and a control oligonucleotide with duplicates. Sequencing of the polysomal and monosomal fraction and the total RNA of each sample. There were 4 biological samples ( duplicates of cells transfected with the targeted oligonucleotide and cells tranfected with the control oligonucleotide), and 12 sequencing samples (monosomal fraction, polysomal fraction and TOTAL RNA of each biological sample)