Contribution of different substrates to mitochondrial H2O2 production in A. aegypti flight muscle.

Values were expressed as mean ± SD of pmol hydrogen peroxide produced/min/mg protein in two different mitochondrial metabolic states using: 10 mM pyruvate + 10 mM proline (Pyr+pro), 20 mM sn glycerol-3 phosphate (G3P), or 10 μM palmitoylcarnitine + 5 mM malate (PC+Mal) followed by 2 mM ADP, 4 μg/mL oligomycin (Oligo), and 2 μM FCCP. For all G3P experiments, measurements were carried out after addition of 0.5 μM rotenone. Statistical analyses were carried out between the groups of different substrates and mitochondrial metabolic state within the same sex, and were performed by using Kruskal-Wallis test followed by a posteriori Dunn´s test (indicated by superscript letters). Significant difference in “Oligo” wasap = 0.0052, relative to Pyr+pro and G3P,bp = 0.0045, relative to G3P. Significant differences in “FCCP” was;c p = 0.0065, relative to G3P;;dp = 0.0016, relative to G3P. Analyses were also conducted between the two mitochondrial metabolic states (oligo vs. FCCP) within the same substrate and sex and were performed by using Mann Whitney test (indicated by superscript symbols). Significant differences in G3P were ** p* p = 0.016 relative to male FCCP. Significant difference in PC+Mal were# p = 0.03 relative to female FCCP;Contribution of different substrates to mitochondrial H2O2 production in A. aegypti flight muscle.