Whole genome survey of the obesity model lean congenic mice B6.C-D7Mit353

We have analyzed the differential expression between the obesity model lean congenic B6.C-D7Mit353 (1) and the background C57BL/6J strains, in four different tissues of male mice: Brain, GWAT (Gonadal White Adipose Tissue), liver and muscle (gastrocnemius). From the 32,381 mouse genes covered by the Applied Biosystems AB1700 microarray system, we identified 330 genes passing FDR at 5% and 188 genes passing FDR at 1%, in any one of the four tissues examined. None of the genes that passed FDR testing were in the congenic donor region. 65 genes from the 8 megabases congenic region were assayed; only six genes (Thrsp, Kctd14, Myo7a, Capn5, Dgat2 and Mtap6) passed statistical significance filtering but not FDR filtering. (1) Diament & Warden 2004: PMID 14569280. Keywords: Congenic mice whole genome gene expression survey Brain, GWAT, liver and muscle RNA were extracted from biological triplicates of the congenic B6.C-D7Mit353 (Brain samples: Br353C1b, Br353C4a, Br353C5b; Liver samples: L571, L409, L552; Adipose samples: GWAT407, GWAT409, GWAT571; Muscle samples: Gas409, Gas407, Gas552) and the background C57BL/6J strains (Brain samples: BrB1b, BrB3b, BrB5b; Liver samples: Lb570, Lb572, Lb573, GWAT572; Adipose samples: GWAT615, GWAT411; Muscle samples: Gas408, Gas615, Gas570). Digoxigenin-UTP labeled cRNA was generated and linearly amplified from 1 ug of total RNA. Array hybridization, Chemiluminescence detection, image acquisition and analysis were performed using Applied Biosystems Chemiluminescence Detection Kit and Applied Biosystems 1700 Chemiluminescent Microarray Analyzer following the manufacturer's protocol.