CRISPRi proliferation dropout screen of EGFR cis-regulatory elements. CRISPRi proliferation dropout screen of EGFR cis-regulatory elements

Here we have used using a pooled proliferation dropout screen with KRAB-dCas9 to determine the contribution of cis-regulatory elements around EGFR to cell fitness in EGFR unamplified (GSC23) and amplified (GBM3565) glioblastoma . Overall design: Pooled CRISPR interference screen using KRAB-dCas9 tiling densely across regulatory elements