Raw data supporting "Candida auris infected zebrafish larvae gives an insight on the virulence factor of the C. auris with aggregate forming phenotype"

This dataset contains the raw data and figures associated with a study investigating the virulence characteristics of various Candida auris strains using the zebrafish infection model. Specifically, it includes: Figure 1: Survival curves of zebrafish embryos infected with different C. auris strains over a 96-hour period, compared to a mock control. Figure 2: Quantitative analysis of fungal burden (CFU/mL) within zebrafish embryos infected with five C. auris strains at multiple timepoints. Figure 3: Assessment of biofilm formation by Candida species. (A) Metabolic activity measured via XTT assay at 24 and 48 hours. (B) Biomass quantification using crystal violet staining at the same timepoints. C. albicans ATCC 90028 was used as a positive control. Figure 7: Evaluation of chitin content in the cell walls of C. auris strains through Calcofluor White staining and confocal microscopy, including quantification of fluorescence intensity. Supplementary Table 1: Enzymatic activity profiles (phospholipase, proteinase, hemolysin) of five C. auris strains, compared with C. albicans ATCC 90028, highlighting their virulence-associated hydrolytic enzyme production. All experiments were independently performed in biological triplicates. The dataset supports the findings presented in the manuscript submitted to Virulence and may be useful for researchers studying fungal pathogenesis and host-pathogen interactions in vivo.本数据集包含一项研究的原始数据与配套图表，该研究借助斑马鱼感染模型，探究了不同耳念珠菌（Candida auris）菌株的毒力特征。具体内容如下： 图1：不同耳念珠菌菌株感染斑马鱼胚胎后，96小时内的存活曲线，并与空白对照组进行对比。 图2：在多个时间点下，5株耳念珠菌菌株感染斑马鱼胚胎后体内真菌负荷（菌落形成单位每毫升，CFU/mL）的定量分析。 图3：念珠菌属生物膜形成能力评估。(A) 于24小时与48小时通过XTT实验（XTT assay）检测代谢活性；(B) 于相同时间点采用结晶紫染色法进行生物量定量。本实验以白色念珠菌（Candida albicans）ATCC 90028作为阳性对照。 图7：通过卡尔科弗卢尔白（Calcofluor White）染色结合共聚焦显微镜（confocal microscopy）技术，评估耳念珠菌菌株细胞壁的几丁质含量，同时包含荧光强度的定量分析。 补充表1：5株耳念珠菌菌株与白色念珠菌（Candida albicans）ATCC 90028的酶活性谱（磷脂酶、蛋白酶、溶血素）对比，着重展示其与毒力相关的水解酶产生能力。 所有实验均独立开展了三次生物学重复。本数据集可支撑投稿至《Virulence》期刊的手稿中的研究结论，亦可作为研究真菌致病机制及体内宿主-病原体互作领域科研人员的参考资料。