Messenger RNA (mRNA) microarray analyses of MRC5 cells and primary lung fibroblasts that were transiently transfected with miR-210 mimic and control

To strategically identify direct miR-210 target genes, we first performed messenger RNA (mRNA) microarray analyses of MRC5 cells and primary lung fibroblasts that were transiently transfected with miR-210 mimic and the control To detect the mRNAs in MRC5 cells and lung fibroblasts treated with miR-210 mimic or pre-miR-NC , 100 ng of total RNA was labelled and hybridized using a SurePrint G3 Human GE v2 8x60K Microarray (Agilent Technologies) according to the manufacturer’s protocol.