The RNA-seq study of Leptolyngbya iicbica LK under nitrogen-limitation and dark stress conditions.

In the second RNA-seq experiment of Leptolyngbya iicbica LK in 2023, total RNA was extracted from sample cultures with the help of the RNA Plant Kit of the NucleoSpin with the reference number of 740949.50, following the manufacturers procedure. Experimental comparisons were designed to capture specific physiological responses under nitrogen-limitation and dark stress conditions. The contrasts N-Plus-D-2hr vs. N-Plus-L-2hr and N-Plus-D-24hr vs. N-Plus-L-2hr represent early dark stress and late dark stress that is the combination of both dark and time or nutrient stress effects, respectively. Nitrogen deprivation effects were examined through N-Minus-L-2hr vs. N-Plus-L-2hr, i.e., early nitrogen stress, and N-Minus-L-24hr vs. N-Plus-L-2hr, i.e., late nitrogen stress. Here, N-Minus indicates the ASN growth medium devoid of the only source of nitrogen, i.e., Sodium Nitrate. To assess the combined influence of nitrogen, darkness, and time-related nutrient stress, N-Minus-D-2hr and N-Minus-D-24hr were compared with N-Plus-L-2hr.