RNA-seq was used to determine the miRNAs expression profiles of Ctrl-EVs and M. bovis NX2-EVs.

In this work, we detect the effect of EVs derived from M. bovis-infected BMEC cells (M. bovis NX2-EVs) on regulating the inflammatory response of BoMac. The results indicate M. bovis-infected BMEC cells promote the inflammatory response in BoMac cells by releasing EVs. We furtherly identify the miRNAs in EVs involved in promoting inflammatory response. Understanding their cargo content will provide new insights into the molecular mechanisms of these respective EVs. Overall design: We extracted total RNA from Ctrl-EVs and M. bovis NX2-EVs,performed gene expression profiling analysis