fluorescenceAggregationImaging_twoColour_npr-1_rep13
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This experiment is part of Fluorescence Aggregation Imaging dataset for the following publication: https://www.biorxiv.org/content/early/2018/11/01/398370 <strong>strain</strong> : OMG2 (green), OMG19 (red) <strong>timestamp</strong> : 20170303 <strong>strain_description</strong> : OMG2: mIs12[myo-2p::GFP]II; npr-1(ad609)X. OMG19: rmIs349[myo3p::RFP]; npr-1(ad609)X. <strong>sex</strong> : hermaphrodite <strong>stage</strong> : adult <strong>media</strong> : NGM agar low peptone <strong>arena</strong> : <strong>style</strong> : petri <strong>size</strong> : 35 <strong>food</strong> : OP50, diluted OD = 0.75, freshly seeded <strong>who</strong> : S. Serena Ding <strong>protocol</strong> : Method in Ding, Schumacher et al. doi.org/10.1101/398370. Alternatively, visit dx.doi.org/10.17504/protocols.io.vzje74n. <strong>lab</strong> : <strong>name</strong> : Behavioural Genomics <strong>location</strong> : London, United Kingdom <strong>software</strong> : <strong>name</strong> : tierpsy (https://github.com/ver228/tierpsy-tracker) <strong>days_of_adulthood</strong> : 1 <strong>total time (s)</strong> : 3600 <strong>frames per second</strong> : 9 <strong>video micrometers per pixel</strong> : 5.128
本实验隶属于用于下述发表论文的荧光聚集成像数据集:https://www.biorxiv.org/content/early/2018/11/01/398370 - 菌株(Strain):OMG2(标记绿色荧光)、OMG19(标记红色荧光) - 时间戳(Timestamp):20170303 - 菌株描述(Strain Description):OMG2:携带mIs12[myo-2p::绿色荧光蛋白(Green Fluorescent Protein, GFP)]转基因插入于II号染色体,且npr-1基因存在ad609突变(位点位于X染色体)。OMG19:携带rmIs349[myo3p::红色荧光蛋白(Red Fluorescent Protein, RFP)]转基因,且npr-1基因存在ad609突变(位点位于X染色体)。 - 性别(Sex):雌雄同体(Hermaphrodite) - 发育阶段(Stage):成虫期 - 培养基(Media):低蛋白NGM琼脂培养基 - 实验载体(Arena): - 样式(Style):Petri培养皿 - 尺寸(Size):35 - 食物(Food):OP50大肠杆菌菌株,经稀释至光密度(Optical Density, OD)=0.75,新鲜接种 - 采集者(Who):S. Serena Ding - 实验方案(Protocol):详见Ding、Schumacher等人的研究方法(doi.org/10.1101/398370),亦可通过dx.doi.org/10.17504/protocols.io.vzje74n获取详细方案。 - 实验室(Lab): - 名称(Name):行为基因组学(Behavioural Genomics) - 地点(Location):英国伦敦 - 软件(Software): - 名称(Name):tierpsy(https://github.com/ver228/tierpsy-tracker) - 成虫培养天数(Days of Adulthood):1 - 总时长(Total Time (s)):3600秒 - 帧率(Frames per Second):9帧/秒 - 视频像素微米换算比(Video Micrometers per Pixel):5.128 微米/像素



