Evaluation of the interaction between <i>Foxe3-</i> (A) and <i>Mip-</i> (B) <i>bicoid</i> sites and proteins in wild-type and –<i>miak</i> mice by electrophoretic mobility assay (EMSA).

EMSA performed with Foxe3- [16] and Mip- [21] bicoid oligonucleotides (oligo probe) and nuclear extracts (NE) from wild-type and miak eyes at E17.5. Although the formation of the specific EMSA complex occurred by combining oligo probes and NEs from wild-type and miak mice, the binding ability was increased with miak-NE and both Foxe3- and Mip-bicoid oligo probes. The binding ability of both oligo probes was inhibited by 10-fold excess unlabeled competitive probes (competitor).