Genome-wide maps of TLR3 activated human keratinocytes compared with control human keratinocytes

In the present study, RNA sequencing of 6 human keratinocytes cell samples was performed. They are 3 samples of Poly (I:C)-treated cells, and 3 samples from un-treated control cells, from 3 different human donors. Total RNA was isolated from the cell samples and one RNA sequencing library was prepared from the 6 RNA samples with the Illumina TruSeq Stranded mRNA HT technolog. This approach uses fragmentation, a poly-T oligo pulldown and sequencing adaptor ligation. RNA sequencing was performed on the Illumina NextSeq500 next generation sequencing system and its high output mode with 1x75 bp single-end red chemistry. Examination of TLR3 activation in human primary keratinocytes on the regulation of gene expression.