Next-generation sequencing measuring mRNA expression in naïve T cells isolated from Mettl3 KO and WT mice
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资源简介：
Naïve T cells were obtained by StemCell CD4+ T cell isolation kit of spleen from Mettl3 KO and WT mice followed by FACS sorting (CD4+CD25-CD45RB-Hi). Raw sequencing reads were aligned to the mouse genome (mm10) with Tophat, and gene expression levels were measured by Cufflinks. Overall design: Mettl3 KO & WT mouse naïve T cells mRNA profiles were generated by deep sequencing, in duplicate, using Illumina HiSeq 2000.
创建时间：
2017-11-17
