Database On The Taxonomical Identification And Potential Toxigenic Capacities Of Non-Qps

The work performed constitutes the external scientific report of the EFSA contract OC/EFSA/FEED/2015/01. The aim of the project has been to provide EFSA with a database from a review on the taxonomical description and potential toxigenic capacities of microorganisms used for the industrial production of feed additives and food enzymes. The review includes microorganisms producing feed additives and food enzymes for which EFSA has received or can potentially receive applications for safety assessment, and which have not been recommended for Qualified Presumption of Safety (QPS) status. The database also comprises the molecular taxonomical identifiers and biosynthetic pathways involved in the production of toxic compounds and responsible genes. The main result of the project is shown as a database according to the EFSA data structure has been developed. The methodological aspects and the queries used in the systematic search and the procedure applied for the screening of retrieved scientific documents are described in this report. Details are available in supplementary appendices to this report. In total, 22970 scientific documents were screened in the literature search from which 411 were initially selected for providing pertinent data for the scope of the project. From the review of the selected articles, 474 bioactive secondary metabolites were recorded and 59 compounds were further studied for obtaining data on their toxicology and characteristic of their production by microorganisms used in industrial fermentations. The database generated in this project comprises details that characterized the conditions, genes involved and toxicity of these 59 compounds. This provides information that can be used to establish safety measures when using potentially toxigenic microorganisms in industrial fermentations. The searching strategy was defined after a preliminary study in which, general information about the fermentative process involving the microorganisms within the scope was obtained. This allowed to identify possible problems that can arise when retrieving data from this heterogeneous group of microorganisms. Several groups of species and groups of keywords were established to perform the searching strategy. The groups of keywords are the following: Keywords group 1: Terms related to toxin production and hazards Keywords group 2: Terms related to feed additives and food enzymes Keywords group 3: Terms related to fermentative processes Keywords group 4: Terms related to toxicology Keywords group 5: Terms related to biosynthetic pathways The microbial species has been divided into 3 groups, <strong>Species I, Species II</strong>, and <strong>Species III,</strong> according to the preliminary outcome in PubMed search: <strong>Species I</strong>: Microorganisms that produced ≤ 200 entries when searched by scientific name. <strong>Species II</strong>: Microorganisms that produced ≤ 500 entries when searched by scientific name and keywords from group <strong>Species III</strong>: Microorganisms that produced &gt; 500 entries when searched by scientific name and keywords from group 1本项目成果为欧洲食品安全局（EFSA）合同OC/EFSA/FEED/2015/01对应的外部科学报告。本项目旨在为欧洲食品安全局（EFSA）构建一套数据库，该数据库源自对工业生产饲料添加剂与食品酶制剂所用微生物的分类学描述及其潜在产毒能力的综述研究。本次综述涵盖了那些已被EFSA受理或潜在可提交安全评估申请、且未被推荐获得合格推定安全（Qualified Presumption of Safety, QPS）资质的、用于生产饲料添加剂与食品酶制剂的微生物。该数据库同时收录了与有毒化合物生产相关的分子分类学标识、生物合成通路及对应的功能基因。本项目的核心成果为一套符合欧洲食品安全局（EFSA）数据规范构建的数据库。本报告详述了系统检索所用的方法学细节与检索式，以及对检索到的科学文献进行筛选的流程；更多细节可查阅本报告的补充附录。 本次文献检索共筛选22970篇科学文献，其中初步遴选411篇，用于为本项目范围提供相关数据。经对遴选文献的综述，共记录474种生物活性次生代谢产物，并针对其中59种化合物展开深入研究，以获取其毒理学数据及工业发酵用微生物的产该类物质的相关特性。本项目构建的数据库收录了这59种化合物的生产条件、涉及基因及毒性特征等详细信息，可为工业发酵中使用潜在产毒微生物时制定安全管控措施提供参考依据。 检索策略的制定基于一项预研究：该预研究获取了本项目范围内涉及微生物的发酵工艺通用信息，从而明确了从这类异质性微生物群体中检索数据时可能遇到的问题。为制定检索策略，本研究划分了多组微生物物种与关键词组，具体关键词组如下： 关键词组1：与产毒及危害相关的术语 关键词组2：与饲料添加剂及食品酶制剂相关的术语 关键词组3：与发酵工艺相关的术语 关键词组4：与毒理学相关的术语 关键词组5：与生物合成通路相关的术语 本研究依据PubMed检索的初步结果，将微生物物种划分为3组：<strong>物种I</strong>、<strong>物种II</strong>及<strong>物种III</strong>： <strong>物种I</strong>：以学名检索时检索结果条目数≤200的微生物。 <strong>物种II</strong>：以学名及对应组关键词检索时检索结果条目数≤500的微生物。 <strong>物种III</strong>：以学名及组1关键词检索时检索结果条目数＞500的微生物。