RNAseq data of duck liver after feeding choline chloride

We aim to study the unusual TMA metabolism mechanism of ducks, and further explore the hidden reasons that led to the weakening TMA metabolism ability. To achieve this, transcriptome, proteome, and metagenome analyses were integrated based on the constructed duck populations with high TMA metabolism ability and low TMA metabolism ability. In addition, further experiments were followed to validate the hypothesis on the limited flavin-containing monooxygenase 3 (FMO3) metabolism ability of ducks. The study demonstrated that both cecal microbe, including Akkermansia and Mucispirillum, and liver FMO3 participated in the TMA metabolism process of ducks. The limited oxidation ability of FMO3 explained the weakening TMA metabolism ability of ducks. Nevertheless, it contributed to the duck’s survival and reproduction during the evolutional adaption process. A population of 294 ducks (Jingjiang, 72 wks) was fed a diet containing 4000 mg/kg choline chloride (TMA precursor), 6 liver samples with low egg TMA levels, 6 liver samples with high egg TMA levels, and 16 random liver samples were selected for RNAseq sequencing