Dataset pHluorin cells experiencing entry into quiescence
收藏资源简介:
Dataset relative to https://www.biorxiv.org/content/10.1101/2020.11.25.395608v2 <strong>Data type</strong>: 2D microscopy images(.tif) <strong>Microscopy data type</strong>: Brightfield images + 2 colors <strong>Imaging</strong>: 60x 1.40 NA brightfield, 6.0µm*6.0µm sCMOS <strong>Cell type</strong>: Budding yeast cells (BY4742) + pHluorin <strong>File format</strong>: .tif (16-bit RGB, 1 color per z-stack). Each folder is a channel <strong>Image size</strong>: 2000x2000 pixels, binned to 2x2 <strong>Author(s)</strong>: Théo, ASPERT; Basile, JACQUEL; Gilles CHARVIN <strong>Contact email</strong>: theo.aspert@gmail.com; charvin@igbmc.fr <strong>Affiliation</strong>: IGBMC, Université de Strasbourg <strong>Funding bodies</strong>: This work was supported by the Agence Nationale pour la Recherche, the grant ANR-10-LABX-0030-INRT, a French State fund managed by the Agence Nationale de la Recherche under the frame program Investissements d'Avenir ANR-10-IDEX-0002-02. <strong>Additional information: </strong>Framerate is indicated in the .rtf file.
本数据集关联于预印本资源:https://www.biorxiv.org/content/10.1101/2020.11.25.395608v2 **数据类型**:二维显微图像(.tif格式) **显微数据类型**:明场成像+双色信号 **成像参数**:采用60倍物镜、数值孔径1.40(NA)的明场光路,使用科学互补金属氧化物半导体(sCMOS)相机采集,像素尺寸为6.0μm×6.0μm **细胞类型**:芽殖酿酒酵母(BY4742菌株)+ pHluorin荧光蛋白(pHluorin) **文件格式**:采用.tif格式,为16位RGB图像,每个z轴堆叠(z-stack)对应一个颜色通道;每个文件夹代表一个成像通道 **图像尺寸**:单张图像分辨率为2000×2000像素,经过2×2像素合并(binning)处理 **作者**:ASPERT Théo、JACQUEL Basile、CHARVIN Gilles **联系邮箱**:theo.aspert@gmail.com;charvin@igbmc.fr **所属机构**:斯特拉斯堡大学IGBMC研究所 **资助机构**:本研究获得法国国家研究署(Agence Nationale pour la Recherche)资助,具体包括项目ANR-10-LABX-0030-INRT,以及由该机构在“未来投资计划”(Investissements d'Avenir)框架下资助的项目ANR-10-IDEX-0002-02 **补充说明**:帧率相关信息详见.rtf格式文件



