Secondary Graft Loss After Third-party "Off-The-Shelf" Antigen-Specific T cell Infusion

Here, we describe a severe adverse event of virus-specific T cell (VST therapy) in an infant with severe combined immunodeficiency who received VSTs for treatment of CMV viremia post-transplant and developed secondary graft rejection at 1-month post-VST infusion associated with the expansion of VST donor-derived T cells. Deposited here are the bulk repertoire, single-cell gene expression and TCR, and flow cytometry data from the study. bulk_rep.zip contains tables of alpha/beta TCR sequences for post-infusion, VST donor, and VST product samples, and samples stimulated with neoepitope peptides. VST_agg_wTCR_SS.rds is an R Data Serialization file containing a Seurat object of the processed 10X single-cell gene expression and TCR profiling for the post-infusion, VST donor, and VST product samples. TCR information is stored in the meta.data slot. P0230D neoantigen expansions.acs, P0230D pentamers.acs, and P0230D_MLR.acs contain the flow cytometry data for the neoantigen and mixed lymphocyte reaction experiments.本研究报道了1例接受病毒特异性T细胞（virus-specific T cell, VST）治疗的重症联合免疫缺陷患儿发生的严重不良事件：该患儿在移植后因巨细胞病毒（cytomegalovirus, CMV）血症接受VST治疗，于VST输注后1个月出现继发性移植物排斥反应，该不良事件与供者来源的VST T细胞扩增密切相关。本数据集收录了本研究产生的批量T细胞受体谱系、单细胞基因表达谱与T细胞受体（TCR）数据以及流式细胞术数据。 bulk_rep.zip 包含输注后样本、VST供者样本、VST制备样本以及新表位肽刺激样本的αβ型T细胞受体序列表格。 VST_agg_wTCR_SS.rds 为R数据序列化文件，内含经处理的输注后样本、VST供者样本及VST制备样本的10×单细胞基因表达与TCR谱分析Seurat对象，TCR相关信息存储于其元数据字段中。 P0230D neoantigen expansions.acs、P0230D pentamers.acs及P0230D_MLR.acs 包含新抗原及混合淋巴细胞反应实验的流式细胞术数据。